Journal: NPJ Science of Food
Article Title: Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice
doi: 10.1038/s41538-024-00359-7
Figure Lengend Snippet: A–C The protein level of total and nucleus Nrf2 in liver. D–F The mRNA expression of antioxidant-related genes in the Nrf2/ARE pathway in liver. * p < 0.05; ** p < 0.01; *** p < 0.001 when compared to CON group; # p < 0.05; ## p < 0.01; ### p < 0.001 when compared to HFD group.
Article Snippet: The primary antibodies (ProteinTech, China) used in the present study included NF-E2-related factor 2 (Nrf2) (1:2000, 80593-1-RR) and β-actin antibody (1:5000, 81115-1-RR) were used for incubation at 4 °C for 10 h. The protein blots were then incubated with the IgG-horseradish peroxidase-conjugated secondary antibody (1:5000, PR30009).
Techniques: Expressing